ABSTRACT
Objective: The mechanism of Gualou Guizhi Decoction (GGD) in the treatment of lower limb spasm after stroke was analyzed based on network pharmacology. Methods: Using the TCMSP and SwissTarget Prediction platform to screen the main components and targets of GGD, the obtained target information was imported into Gephi software to build the active components-targets network of GGD. Genecard, DisGeNET, and TTD disease databases were used to screen target genes for stroke and lower limb spasm; Target protein interaction network was constructed by using STRING platform; KEGG pathways, and GO (Gene Ontology) function were analyzed by using ClueGO software and DAVID v 6.8 platform. Results: Flavonoids, organic acids, and saponins of GGD was applied to 49 key target genes, such as IL6, TNF, EGFR, ESR1, TP53, STAT3, and F2, etc; Forteen category biological processes including proliferation of striated muscle cells, bone remodeling, smooth muscle cells, muscle cells and endothelial cells proliferation were involved; Thirty signaling pathways, including TNF, MAPK, PI3K-Akt, ALS, serotonergic synapse, and neurotrophin, etc, were regulated. Conclusion: Various active ingredients of GGD has pharmacological effects such as anti-inflammatory, antioxidant, neurotrophic, and participate in some biological processes, such as muscle cell proliferation, so as to treat lower limb spasm after stroke from central nervous system and peripheral muscle tissue two-ways.
ABSTRACT
Objective@#To observe the effect of modified Gualou-Guizhi decoction combined with hyperbaric oxygen on muscle tension and motor function in stroke patients with spastic hemiplegia.@*Methods@#A total of 70 stroke patients with spastic hemiplegia met the inclusion criteria were randomly divided into the observation group and the control group, with 35 in each group. The control group was given the conventional treatment and hyperbaric oxygen treatment, while the observation group was treated with Gualou-Guizhi decoction on the basis of the control group. Lindmark scale was used to assess limb motor function, Fugl-Meyer Assessment Scale (FMA) was used to assess the degree of limb motor dysfunction, and Modified Ashworth Scale (MAS) was used to assess muscle tension. The root mean square (RMS) and frequency median (FM) of surface electromyography of quadriceps femoris were measured by automatic electromyography, and the clinical efficacy was evaluate.@*Results@#The total effective rate of the observation group was 91.4% (32/35), and the total effective rate of the control group was 71.4% (25/35). The total effective rate of the 2 groups was statistically significant (χ2=4.629, P=0.0314). After treatment, the scores of motor coordination ability and sensory function in the observation group were significantly higher than those in the control group (t value were 5.936, 5.182, all Ps<0.05), the FMA scores of upper and lower limbs were significantly higher than those in the control group (t value were 12.310, 11.996, all Ps<0.01), and the score of improved Ashworth muscle strength scale was significantly lower than that in the control group (t=5.083, P=0.031). After treatment, the EMG RMS (79.13 ± 7.02 vs. 68.45 ± 7.01 , t=5.697), FM (99.31 ± 9.65 Hz vs. 90.73 ± 9.49 Hz, t=5.382) of the observation group were significantly higher than those of the control group (P<0.05).@*Conclusions@#The application of Gualou-Guizhi decoction add and subtract combined with hyperbaric oxygen on muscle tension and motor function in stroke patients with spastic hemiplegia can improve the muscle strength of stroke hemiplegic patients and improve the motor dysfunction of the patients, which can help to promote the recovery of the movement function of the patientst.
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Objective: To study the serum pharmacochemistry of Gualou Guizhi Decoction (GLGZD) as well as the material basis through analyzing the constituents absorbed in blood. Methods: A high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (HPLC-Q-TOF-MS) method was developed for the analysis on absorbed components in serum after ig administration of GLGZD to rats. The separation was achieved on a C18 column by gradient elution with acetonitrile-aqueous solution (containing 0.1% formic acid) as mobile phase. The ion acquisition was performed by full scan MS and MS/MS in negative ion mode.The serum after ig administration of GLGZD to rats, blank serum of rats, and GLGZD extracts sample were analyzed by HPLC-Q-TOF-MS. Results: Forty-two prototype compounds including monoterpene glycosides, flavonoids, phenolic acids, gingerols, triterpenoid saponins, galloylglucoses, and others were confirmed and identified in serum by comparing the retention time and mass spectrometry fragmentation characteristics with reference standards or literatures. Conclusion: The analysis of absorbed components of GLGZD in serum after administration by serum pharmacochemistry method would provide an experimental basis for revealing the real neuroprotective constituents of GLGZD in treating dysfunction after stroke.
ABSTRACT
The paper was aimed to establish a quality evaluation model for Gualou Guizhi decoction based on the chemical compositions and biological effects. Ultra high performance liquid chromatograph-mass spectrometer was used to analyze and determine 24 kinds of chemical compositions in Gualou Guizhi decoction, and then, biological activity effect was quantitatively assessed in a zebrafish neuronal injury model which was induced by mycophenolate mofetil (MMF). As a result, the established method for quality evaluation of Gualou Guizhi decoction based on the chemical compositions and biological effects is feasible, stable and reliable, which can provide reference for quality control of compound Chinese medicines.